Prevention effect of lower extremity deep venous thrombosis with A-V impulse foot pump for patients who took spinal surgery / 中国实用护理杂志

Objective To explore the application of A-V impulse foot pump in patients with lumbar spine surgery to prevent lower extremity deep venous thrombosis. Methods A total of 139 patients with spinal lesions who took spinal surgery treatment from April 2015 to March 2016 were divided into experimental group (69 cases) and control group (70 cases) by random digits table method. Control group was given normal nursing instruction, and the person who had negative results of B ultrasonic examination in experimental group was accepted physical therapy with A-V impulse foot pump. The patients got secondary ultrasound or CT examination who had the deep venous thrombosis symptoms and/or highly suspected deep venous thrombosis in order to further confirmed, then calculated the incidence rate of two groups with deep venous thrombosis. Results There were 4 cases of 139 patients who got deep venous thrombosis in 2-6 days of postoperative, 1 case in experimental group, 3 case in control group, compared with control group, patients who used A-V impulse foot pump had lower risk of deep venous thrombosis, but there was no significant difference (P>0.05). The operation time, body mass index were (5.00 ± 0.82) h , (30.54 ± 3.93) kg/m2 in patients with deep venous thrombosis and (3.16 ± 0.66) h, (24.90 ± 2.62) kg/m2 in patients without deep venous thrombosis, and there were significant differences(t=5.44, 4.19, P<0.01). Conclusions The application of A-V impulse foot pump has positive significance to prevent the happening of deep venous thrombosis for patients who took spinal surgery, and it is worth to be popularized and applied in clinic.

Prevention effect of lower extremity deep venous thrombosis with A-V impulse foot pump for patients who took spinal surgery / 中国实用护理杂志

Objective To explore the application of A-V impulse foot pump in patients with lumbar spine surgery to prevent lower extremity deep venous thrombosis. Methods A total of 139 patients with spinal lesions who took spinal surgery treatment from April 2015 to March 2016 were divided into experimental group (69 cases) and control group (70 cases) by random digits table method. Control group was given normal nursing instruction, and the person who had negative results of B ultrasonic examination in experimental group was accepted physical therapy with A-V impulse foot pump. The patients got secondary ultrasound or CT examination who had the deep venous thrombosis symptoms and/or highly suspected deep venous thrombosis in order to further confirmed, then calculated the incidence rate of two groups with deep venous thrombosis. Results There were 4 cases of 139 patients who got deep venous thrombosis in 2-6 days of postoperative, 1 case in experimental group, 3 case in control group, compared with control group, patients who used A-V impulse foot pump had lower risk of deep venous thrombosis, but there was no significant difference (P>0.05). The operation time, body mass index were (5.00 ± 0.82) h , (30.54 ± 3.93) kg/m2 in patients with deep venous thrombosis and (3.16 ± 0.66) h, (24.90 ± 2.62) kg/m2 in patients without deep venous thrombosis, and there were significant differences(t=5.44, 4.19, P<0.01). Conclusions The application of A-V impulse foot pump has positive significance to prevent the happening of deep venous thrombosis for patients who took spinal surgery, and it is worth to be popularized and applied in clinic.

Clinical Efficacy Analysis of Naloxone Combined with Edar-avone on Acute Alcoholism / 昆明医科大学学报

Objective To study the clinical efficacy of naloxone combined with edaravone on acute alcoholism. Methods One hundred and twenty-five patients with acute alcoholism were randomly divided into the control and treatment groups. The control group accepted conventional treatment and taking naloxone, while the treatment group accepted edaravone on the basis. Before and after treatment,the treatment recovery time,recovery respiratory rate time and symptom disappearance time were recorded. The malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), neuron-specific enolase (NSE), S-100βand vascular cell adhesion points-1 (VCAM-1) were detected. Results After treatment,the recovery time and recovery respiratory rate time of the treatment group were significantly shorter than those of the control group ( <0.05) . In the control group, S-100β was significantly improved after treatment ( <0.05) . NSE, S-100β, VCAM-1 and MDA have significantly decreased ( <0.05) after treatment in the treatment group. Compared with the control group, there were significant differences of S-100β,VCAM-1 and MDA in the treatment group before and after treatment (<0.05) . While SOD and GSH have significantly increased ( <0.05) after treatment and showed significant differences compared with the control group ( <0.05) . Conclusion Naloxone and edaravone combination therapy can improve oxidative stress and nerve damage, which help early rehabilitation in patients with acute alcoholism.

Fluorescence spectra and test application study on the interaction of fluorescein and bovine serum albumin / 生物医学工程学杂志

A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex exitation wavelength is 467 nm, the emission wavelength is 515 nm. The linear measurement rang is between 1.8-500 mg/L for BSA, F = 26.776 C + 2.8082, r = 0.9999. This method is sensitive,steady,and low cost for determination of BSA.

Change of carbon monoxide in plasma and tissue during acute hypoxia / 中国医学科学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the role of endogenous carbon monoxide (CO) in hypoxia.</p><p><b>METHODS</b>After rats were inhaled with hypoxic gases and the heme oxygenase inhibitor ZnPPIX was administered, we measured the CO levels in plasma, liver, lung and kidney. Meanwhile plasma cGMP levels were observed. Furthermore, we recorded the change of hemodynamic and blood gases.</p><p><b>RESULTS</b>Acute mild hypoxia (10% O2) significantly increaed CO levels in plasma as well as liver, kidney and lung, while acute severe hypoxia (5% O2) significantly decreased CO levels in plasma as well as liver, kidney and lung. In addition, the former significantly elevated cGMP levels in plasma while the latter markedly reduced cGMP levels in plasma. The hemodynamic change occurred in accordance with the changes carbon monoxide.</p><p><b>CONCLUSION</b>Our results indicate, for the first time, that the endogenous carbon monoxide plays an important role in regulating the vessel tone during hypoxia.</p>

Effect of Anisodamine on Myocardial Reperfusion Injury / 中国医科大学学报

Objective: Our purpose was to study the effect of anisodamine on the injury of myocardium after myocardial ischemia reperfusion. Methods: Eighteen patients (ASA grade Ⅱto Ⅲ) scheduled for valve replacement, were randomly divided into 2 groups during extracorporeal circulation. Anisodamine (0.25 mg/kg) was given intravenously in the anisodamine group before reperfusion. Equivalent volumes of normal saline were administered in the control group. The central venous blood samples were collected at different time after ischemia and reperfusion. We then measured the concentrations of lactic dehydrogenase (LDH), creatine kinase (CK), and malondiadehyde (MDA) with biochemical methods. Results: The levels of serum LDH, CK, and plasma MDA increased significantly in the control group during ischemia and reperfusion. In the anisodamine group, the levels of serum LDH and CK increased. But the values were lower at corresponding time than those in the control group (P<0.05) during reperfusion. The plasma level of MDA unchanged in the anisodamine group during ischemia and reperfusion. The postoperative cardiac function recovered much more better in the anisodamine group. Conclusion: Anisodamine can reduce the degree of reperfusion injury. A certain protective effect on myocardial ischemia reperfusion injury exsists during valve replacement.

Effects of hyperventilation on the balance of cerebral oxygen supply and consumption during craniotomy / 中华麻醉学杂志

Objective To assess the effects of hyperventilation on the balance of cerebral oxygen supply and consumption during craniotomy Methods Twenty ASA Ⅰ Ⅱ patients with supratentorial tumor undergoing elective craniotomy were studied Anesthesia was induced with fentanyl 2 4 ?g/kg and propofol 1 2mg/kg I V and tracheal intubation was facilitated with succinylcholine 1 5 2 0mg/kg I V After tracheal intubation the patients were mechanically ventilated Anesthesia was maintained with isoflurane with or without nitrous oxide and intermittent intravenous bolus of vecuroinum The patients were randomly divided into two groups : in group Ⅰ anesthesia was maintained with 1 5% isoflurane alone (ISO group, n=10); in group Ⅱ with 0 8% isoflurane and 60% 65% N 2O(ISO N 2O group, n=10) Demographic data including age, sex and weight were not significantly different between the two groups In group Ⅰ there were 6 male and 4 female, the mean age was (40 1?9 7) years and weight (64 5?7 3)kg whereas in group Ⅱ there were 5 male and 5 female, mean age was(43 6?8 4)years and mean body weight (62 7?6 5)kg After the opening of dura mechanical ventilation was regulated to maintain PaCO 2 at 4 7 6 0kPa(normal ventilation), 0 9) Arterial and venous blood lactate was within normal range but had a tendency to increase Conclusions Severe hyperventilation influences the balance of cerebral oxygen supply and consumption significantly during either isoflurane or isoflurane N 2O anesthesia at equal MAC, particularly in ISO N 2O group Severe hyperventilation should not be used during anesthesia for brain surgery

Time course of potentiation of vecuronium by sevoflurane and isoflurane / 中华麻醉学杂志

To observe the time-dependent course of potentiation of vecuronium produced by 1MAC of end-tidal sevoflurane and isoflurane. Method: At the beginning, a steady infusion rate of vecuronium to maintain 90 of neuromuscular block was established in 40 patient undergoing neurosurgical procedures under propofol fentanyl nitrous oxide oxygen anesthesia. The patients were randomly assigned to receiving IMAC end-tidal concentration of either sevoflurane (Group Sev) or isoflurane(Group Iso). Vecuronium infusion rate was adjusted to maintain 90%, neuromuscular block. The change of infusion rate of vecuronium with time was observed after inhalation of Sev or Iso. Result: Sev and Iso decreased the infusion dosage of vecuronium in an exponential manner, maximal potentiation occurred 90 min after inhalation. Maximal reduction in infusion rate was 67.87% in group Sev and 69.87% in group Iso without the significant difference between them. Conclusion: Sev and Iso can potentate the muscular relaxation of vecuronium at similar degree in strong time-dependent way.